Breakthrough in DNA Analysis Technology.

Our sensors allow quick and cost-effective detection of changes in NA structure.

Advantages

It simplifies workflows without fluorescent detection or PCR, enabling faster, more accessible diagnostics in healthcare, agriculture, and beyond.

Sensitivity

Comparable to PCR with simplified workflows, without fluorescent detection.

Speed

Delivers precise results in under 30 minutes.

Portability

Compact and ready for both lab and field use.

Versatility

Adaptable for various applications, from healthcare to agriculture.

Customizability

Tailored solutions to meet specific diagnostic needs, without the need for matrix enrichment.

How it works

How the Nucleotica
nanosensor platform works.

The Nucleotica Nanosensor Platform is an innovative diagnostic solution that utilizes advanced electrochemical biosensor technology for fast, precise, and reliable DNA detection.

The streamlined process ensures accuracy while remaining simple for diverse applications in clinical and research environments. Here is a step-by-step explanation of the platform's functions:

01 Sample preparation.
This step makes DNA detectable.

Types of samples: Blood, saliva, tissue, or environmental samples.
DNA extraction: DNA or RNA is extracted from the sample using standard techniques.
Preparation is quick, taking just a few minutes.
Minimal equipment: Preparation is simplified, reducing the need for complex devices or training.

02 Hybridization.
Specific binding DNA to a probe.

Electrodes are coated with short, specific DNA probes for target sequences.
Probes ensure desired DNA detection, minimizing false results.
Hybridization: The sample binds target DNA to the probe, forming a stable duplex.
Flexibility: The platform customizes detection of genetic markers, mutations, or pathogens.

03 Electrochemical reaction.
Transforms interaction into electrical signal.

Target DNA binding changes electrode's electrochemical properties like resistance or conductivity.
Sensors function reliably with standard PCR inhibitors and with degraded DNA, ensuring performance.
Nanosensor captures changes, converting biochemical events to electrical signals.
Instant reaction ensures quick results, no long waits.

04 Data processing.
Nanosensor signals processed with the software.

Electrochemical signals are digitized for analysis.
The platform confirms and quantifies target DNA, offering concentration insights.
Proprietary software analyzes data, ensuring accuracy.
Quality control and algorithmic corrections minimize errors.

Nanosensor mechanism

Nucleotica innovates DNA & RNA analysis with nanosensors.

Carbon nanotube electrodes.

The sensors are modified with carbon nanotubes to enhance sensitivity and reliability. This allows for precise detection of nucleic acids, even in degraded or complex samples.

Short oligonucleotide probes.

Unlike traditional methods, Nucleotica uses unmodified oligonucleotide probes that can be as short as 20–40 base pairs. These probes bind specifically to target sequences, initiating an electrochemical signal upon hybridization.

Electrochemical parameter changes.

The hybridization of the target DNA with the probe DNA results in measurable changes in the sensor's electrochemical properties, providing a direct and rapid signal.

Exclusive technological expertise

This design removes fluorescence detection or PCR, simplifying analysis with high accuracy.

Customizable panels.

The platform offers tailored solutions for specific needs. Whether it's for oncology, virology, or forensic science, users can design probes and panels suited to their unique applications.

Simplified workflow.

Nucleotica's process requires only basic laboratory skills, making it accessible for a wide range of users. The device operates with minimal preparation, focusing on isolating DNA or RNA before analysis.

Durability and portability.

The device is resistant to mechanical vibrations and designed for field testing. It does not require optical detection components, ensuring reliability in diverse environments.

Achievements and testing

Comparison
with traditional methods.

Efficiency.

DNA analysis can be complex with PCR and optical detection. Nucleotica simplifies this, saving time and costs.

Accuracy.

The platform works with challenging samples like forensic evidence or fragmented DNA, ensuring accurate results.

Sensitivity.

Capable of detecting DNA across ten orders of magnitude.

Specificity.

Significantly reduced false positives compared to fluorescence-based methods.

Why choose Nucleotica?

Safety and environmental impact.

The absence of chemical reagents and lasers enhances personnel safety and simplifies disposal, while durable materials and a long device lifespan reduce environmental impact.

Versatility.

Suitable for clinical diagnostics, research labs, agriculture, and more.

Cost Efficiency.

Advanced technologies make high-precision analysis more affordable, reliable, and cost-effective.

Adaptability.

Nucleotica can rapidly develop panels tailored to any customer request, enhancing workflow speed, laboratory flexibility, and swift adaptation to new challenges.

Analysis Speed.

Eliminating the DNA/RNA amplification step significantly reduces analysis time (to 15–30 minutes) while maintaining high accuracy.

Sensitivity and specificity.

Nucleotica technology offers sensitivity comparable to digital PCR and extremely high specificity.

Where is it used?

We create advanced equipment to enhance precision and efficiency in diagnostics and healthcare.

Laboratories.

Research labs and diagnostic facilities: We equip, furnish, and enhance any laboratory in a short time, boosting its capabilities with our advanced equipment.

Clinics.

Hospitals and healthcare centers: We strengthen clinics with our advanced equipment and unique tests, enabling them to solve scientific and diagnostic challenges more efficiently.

Educational institutions.

Universities and training programs: We enhance and expand the educational and research toolkit for educators and students with our equipment.

Research institutes.

Specialized organizations focused on advanced studies: We empower research teams with our user-friendly equipment, software, and revolutionary technology, driving innovation and efficiency.

Additional areas of application

The technology’s adaptability and precision make it invaluable across diverse fields.

Clinical diagnostics.

Identifying genomes of wide range of pathogens, monitoring biomarkers, and detecting genomes.

Agriculture.

Early detection of plant pathogens, ensuring crop health.

Transplantology.

Monitoring of minor quantities of donor DNA in recepient blood to detect graft rejection on early stage.

Environmental.

Tracking biodiversity and contamination in ecosystems.

Food safety.

Authenticity testing and pathogen detection in food products.

Let’s discuss

Device in action

See how our Sens 6 device works, testing for KRAS gene mutations

Learn more

Contact us

Reach out to start your next innovation today.

Publications

Explore our groundbreaking research published in leading journals and presented at international conferences.

A single-molecule label-free identification of single-nucleotide colorectal-cancer-DNA polymorphism using impedance spectroscopy of self-redox-active decorated carbon nanotubes

Semiconductors, Vol. 54, no. 14, p. 1873–1876 (2020)

V.P. Egorova, H.V. Grushevskaya, A.S. Babenka, R.F. Chakukov, N.G. Krylova, I.V. Lipnevich, E.V. Vaskovtsev.

Nanopore-Penetration Sensing Effects for Target DNA Sequencing via Impedance Difference Between Organometallic-Complex-Decorated Carbon Nanotubes with Twisted Single-Stranded or Double-Stranded DNA

Advanced Nanomaterials for Detection of CBRN. NATO Science for Peace and Security Series A: Chemistry and Biology. / J. Bonca, S. Kruchinin (eds.) Springer, Dorchester, Chapter 17, p. 247-258 (2020)

A. S. Babenko, H. V. Grushevskaya, N. G. Krylova, I. V. Lipnevich, V. P. Egorova, R. F. Chakukov

Carbon nanotubes as a high-performance platform for target delivery of anticancer quinones

Current Pharmaceutical Design, Vol. 24 (43), p. 5207–5218 (2018)

H.V. Grushevskaya, N.G. Krylova